Pune BioCluster

Bio-Imaging Facility at NCCS, under the Pune-BioCluster initiative, aims to establish a platform with the state-of-the-art imaging capabilities. The centre has already procuredtwo microscopes and in the process of getting three more with diverse capabilities to cater to the needs of scientists from academic and private research laboratories. Once all the microscopes are installed, these will be available for outside users on a chargeable basis. It also envisages the importance of developing highly-skilled manpower by conducting regular national level and local workshops to train researchers with advanced microscopic techniques including advanced light microscopy, confocal microscopy, digital image processing of microscope images, and related laboratory practices.
The facility currently has fiveconfocal laser microscopes. These are available for outside users on a chargeable basis.

• Will provide a state-of-the-art facility for catering to the needs of researchers with diverse imaging capabilities
• Will conduct regular national level and local training programmes to develop skilled manpower in Bio-imaging

NCCS Bio-Imaging Facility Instruments:

Pune Biocluster Project funded:

1. Zeiss LSM880 Confocal Microscope with AiryScan and ELYRA P.1
This imaging system comprises of AxioObserver 7 microscope with AiryScan and Elyra P.1 technology on the LSM 880. Airyscan is a 32-channel GaAsP-PMT area detector that collects a pinhole-plane image at every scan position. Each detector element functions as a single, very small pinhole. ELYRA P.1 localizes small structures and even single molecules achieving resolutions of 20nm laterally and 50nm axially

2. Olympus SPIN SR 10 - Spinning Disk High Resolution Microscope
This is a high speed spectral confocal microscope for macro to microscopic imaging with 3D FRET, FRAP, Photo activation applications.It can attain high resolution nearly 140 nm or better (XY) and 350 nm in Z direction for at least two colours simultaneously with OSR (Olympus Super resolution- software based). Objectives are 1.25X to 60X. ZDC option (Z Drift Compensators) avoids minor vibrations during live imaging and retains the same focus position in real time for long term time lapse imaging. Images can be acquired at high speed or high scan format. It is equipped with a fully motorized XYstage inverted microscope with anti-vibration platform. The system has 4 detectors- 2 PMT detectors and 2 High sensitivity detectors (GaAsP) with possibility of 8 to 16 channels and dedicated transmitted light detector for DIC imaging.

Instruments being procured under Pune Biocluster Project:

1.Structured Illumination microscope and localization based super-resolution platform
2. PSF engineering based super-resolution microscope
3. Multiphoton Fluorescence microscope

Instruments Available at the Facility (NCCS funded):

1. Leica SP5 II Confocal Microscope
This is a high-end Broadband Confocal Laser Scanning Microscope with 3 PMT's, 2 Hybrid detectors and AOBS technology. The microscope is equipped with CO2 incubation chamber for live cell experiments as well as FRET, FRAP experiments and routine multi dye imaging. This is fully motorized, automated and computer controlled microscope Leica DMI 6000. Objectives available are 10x, 20x, 20x multi-immersion, 40x oil, 63x oil, 100x oil.

2. Olympus FV3000 Confocal Microscope
This is a high speed spectral confocal microscope for macro to microscopic imaging with 3D FRET, FRAP, Photo activation applications.It can attain high resolution nearly 140 nm or better (XY) and 350 nm in Z direction for at least two colours simultaneously with OSR (Olympus Super resolution- software based). Objectives are 1.25X to 60X. ZDC option (Z Drift Compensators) avoids minor vibrations during live imaging and retains the same focus position in real time for long term time lapse imaging. Images can be acquired at high speed or high scan format. It is equipped with a fully motorized XYstage inverted microscope with anti-vibration platform. The system has 4 detectors- 2 PMT detectors and 2 High sensitivity detectors (GaAsP) with possibility of 8 to 16 channels and dedicated transmitted light detector for DIC imaging.

3. Thermo CellInsight CX7 LZR - High Content Screening System
This is a fast, laser-based, automated cellular imaging and analysis platform for quantitative microscopy and phenotypic screening. It is a 7-channel, laser-based illumination system with Software and laser-based autofocus for consistent scan times. The objectives range from 2x, 10x, 20x, 40x to 60x. Major applications are fluorescent, confocal and 5-channel brightfield imaging. It also has an onstage incubator for live cell imaging. It is highly compatible with standard microplates (6, 24, 48, 96, 384, 1536 wells). It is loaded with HCS Studio software for integrated data collection and analysis. The system provides powerful statistical method to carryout various bioapplications like- Autophagy assay, Cell viability assay, Kinetic assays, Apoptosis, DNA damage, Transfection efficiency, Cell toxicity and colony formation assays etc

User Charges:

Charges based on number of slots used where one hour is considered as one slot.
Users from other educational institutions: Rs.2000/- per slot + 18% GST.
Users from Industry: Rs.3000/- per slot + 18% GST.
Download Sample Request Form: Download form from here

NCCS Bio-Imaging Facility offers the following services:

• Expert consultation through the Facility In-Charge and our technical specialists.
• Imaging samples in live and fixed conditions including microbial cultures, mammalian cells and nanoparticles.
• Imaging cellular organelles to tissue specimens in live and fixed conditions.
• Assistance with data analysis which can be customized to the needs of individual investigators and research projects.

NCCS Bio-Imaging Facility offers the following trainings on regular basis:

• Hands-on training on Light Microscopy Techniques.
• Training /Workshop on Confocal microscopes, Image and data analysis.
• Training on different Image processing and analysis softwares

Contact

Phone Number: +91-20-2570 8265/8260

E-Mail : bioimaging@nccs.res.in

Address:

Bio-Imaging Facility,
National Centre for Cell Science,
NCCS Complex, P.B. No. 40,
Ganeshkhind Post,
S. P. Pune University Campus,
PUNE – 411 007, Maharashtra State, India.

Physical Location:
Ground floor of New Building at NCCS.