PROTEOMICS FACILITY

About

The Pune Bio-cluster Proteomics Facility aims to provide proteomics and mass spectrometry services including protein identification, proteome analysis, quantitative proteomics, intact mass analysis, phosphoproteomics, metabolites analysis etc. Facility is currently in the process of procuring advanced, state-of-the-art, high throughput mass spectrometry instrumentation. Once we set-up the facility, we are ready to provide the proteomics and mass spectrometry services and this information will be updated on the website in due course of time. In addition to the above services, the facility organizes training programs, workshops etc. to generate highly skilled manpower to undertake cutting edge scientific pursuits.

Salient Features

State-of-the-art high throughput Mass Spectrometers capable of performing high resolution accurate mass determination of the analytes. This instrumentation facility is capable of performing quantitative proteomics analysis, PTMs analysis, labeled and label-free proteomic analysis, top-down proteomics, Metabolite identification and quantification, GC-MS analysis etc.

Instruments procured for the Pune Biotech Cluster Proteomics facility at NCCS


TripleTOF®6600+ LC-MS/MS System



QTRAP® 6500+ LC-MS/MS System

Services

• Currently available: At present, services for MALDI-ToF/ToF mass spectrometry for proteomics (protein identification and intact mass analysis) and Gas Chromatography mass spectrometry for metabolites/small molecules analysis is available at the facility.

Sl.No Service Academic Private
1 LC-MS/MS analysis, [6500 Q-TRAP] 5000 7000
2 LC-MS/MS proteome analysis (digested sample) [TripleToF 6600] 7000 10000
3 LC-MS/MS proteome analysis (including digestion) [TripleToF 6600] 10000 13000

Training

• National workshop on Proteomics 2024 (18-20, November), trained 12 researchers
• Internal workshops on Proteomics 2024 (June, July, August, December), trained 27 researchers

Proteomics training planned ahead
• In-house Hands-on proteomics workshop in Aug-2025
• In-house Hands-on proteomics workshop in Sept-2025
• In-house Hands-on proteomics workshop in Oct-2025
• National proteomics workshop in November-2025